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KMID : 0644120110240020001
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Journal of Oriental Gynecology
2011 Volume.24 No. 2 p.1 ~ p.12
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Antibacterial Effects of Chungdae-tang Aqueous Extracts, and Their Combination Effects with Clindamycin against Gardnerella vaginalis In Vitro
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Kwon Ji-Myung
Kim Dong-Chul
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Abstract
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Objectives:The object of this study was to observe the in vitro antibacterial effects of Chungdae-tang aqueous extracts, traditionally used for treating various gynecological diseases including vaginitis in Korea against Gardnerella vaginalis, and combination effects of Chungdae-tang extracts with Clindamycin were also monitored in this study.
Methods: Antibacterial activities against Gardnerella vaginalis of Chungdae-tang aqueous extracts were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were also monitored at MIC and MIC2 levels. The combination effects of Chungdae-tang aqueous extracts with Clindamycin were observed by Checkerboard microtiter assay, and the effects of bacterial growth curve treated with or Chungdae-tang aqueous extracts MIC+Clindamycin MIC, 1/2MIC and 1/4MIC, respectively. In the present study, Gardnerella vaginalis were incubated under 37℃, 10% CO₂; and bacterial growth curves were calculated at 24, 48, 72, 96 and 120hrs after incubations.
Results: MIC of Chungdae-tang aqueous extracts against Gardnerella vaginalis were detected as 3.906±2.344(0.782~6.250) mg/㎖, respectively. MIC of Clindamycin was detected as 0.010±0.006(0.004~0.016)㎍/㎖ at same conditions. In addition, Clindamycin and Chungdae-tang aqueous extracts also showed marked dosage-dependent inhibition of bacterial growth, and more dramatical inhibitions were detected in Clindamycin+Chungdae-tang aqueous extracts MIC treatment as compared with each of single Clindamycin MIC and Chungdae-tang aqueous extracts MIC treatments, respectively. In addition, quite similar inhibitory effects on bacterial growth were detected in Clindamycin 1/4 MIC+Chungdae-tang aqueous extracts MIC treatment as compared with single Clindamycin MIC treatment in the present study. FIC index in combination of Chungdae-tang and Clindamycin were detected as 0.775±0.285(0.500~1.250) at Checkerboard microtiter assay.
Conclusions : The results obtained in this study suggest that Chungdae-tang aqueous extracts showed antibacterial effects against Gardnerella vaginalis, and it also showed dosage-dependent inhibitory effects on the bacterial growth. In addition, combination treatment of Chungdae-tang aqueous extract with Clindamycin showed more potent inhibitory effects on the growth of Gardnerella vaginalis with FIC index 0.775±0.285(0.500~1.250), respectively. It means, the combination of Chungdae-tang aqueous extract with Clindamycin is partially synergistic effects. It, therefore, is expected that effective dosages of Clindamycin will be reduced to 1/4 or over 1/4 levels as combination with Chungdae-tang extracts, respectively.
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KEYWORD
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Gardnerella vaginalis, Chungdae-tang, Clindamycin, MIC, synergistic effects
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